Analytical Ultracentrifugation (AUC) Service

What is Analytical Ultracentrifugation (AUC)?

Theodor (Thé) Svedberg invented the analytical ultracentrifugation (AUC) method in the 1920s, and it has now become a versatile biophysical method commonly used for in vitro analysis of proteins, nucleic acids and their complexes. Analytical ultracentrifuge is an integration of ultracentrifuge and optical detection system, which can detect the evolution of particle concentration distribution after centrifugation in real time. Analytical ultracentrifugation methods, namely sedimentation velocity (SV-AUC) and sedimentation equilibrium (SE-AUC) methods, are based on the principle that molecules in solution settle at a certain velocity when they are subjected to centrifugal force. Scientists analyze the correlation between sedimentation behavior and f hydrodynamic properties, analyze small peptides and study macromolecular interactions to determine many key parameters of products (such as bulk drugs or drugs).

Figure 1. Illustration of the principle of Analytical Ultracentrifugation (Noy, E.; et al. 2012)

AUC Platform in Creative Proteomics

We have accumulated nearly 20 years of experience in SV-AUC and SE-AUC, and our services cover a variety of applications such as peptides, proteins, viruses, and nanomedicine, etc. We introduce the most advanced AUC technology and professional technical personnel, and are equipped with first-class testing methods. Our team has provided a large number of laboratories and enterprises with high-quality AUC analysis, summarized and developed a powerful method, which can be used for many samples and applications. In addition, in order to meet the different research needs of customers, we also provide customized one-stop services.

Feature Service:

With our state-of-the-art facilities and expertise in analytical ultracentrifugation, we offer comprehensive and high-resolution analysis of AAV vectors to support gene therapy research and development. Our AUC platform enables accurate quantification and characterization of different AAV vector populations, including intact, empty, and partially filled vectors, providing valuable insights into vector purity, shell-to-core ratio, and physical properties. Our experienced team of scientists utilizes advanced ultracentrifuges and detection systems to generate precise density profiles and deliver reliable data for optimizing vector production processes, ensuring consistent vector quality, and advancing the field of gene therapy.

Applications of AUC Assay

• Biopharmaceutical characterization (molecular weight determination, size distribution, diffusion coefficient, sedimentation coefficient, etc.)
• Protein binding and macromolecular interactions (KD, stoichiometry, B22)
• Protein-protein interaction with fluorescent tracers
• Characterization of drug delivery systems
• Aggregate quantification

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Creative Proteomics is an international biotechnology company dedicated to research in molecular interactions and other related fields. The analytical ultracentrifugation platform we constructed has the characteristics of high quality and efficiency, and the data obtained can be directly used for paper publication. Our one-stop service aims to save customers time and money.