Biolayer Interferometry (BLI) Service

Introduction of Biolayer Interferometry (BLI)

Biolayer interferometry (BLI) is a label-free optical technology used to measure intermolecular interactions. It has the advantages of high sensitivity, high throughput, and low vibration/mechanical noise. This technology uses a biosensor with a coated tip (biological layer), immobilizes the bait molecule on the sensor, and then exposes the biosensor tip to buffer conditions and light to monitor the target molecule in real time by recording changes in light interference. The interaction between the analyte and the bait is directly related to the change in the thickness of the biological layer caused by the association of the analyte. The change in the refractive index of the surrounding medium, the change in flow rate, and unbound molecules will not affect the interference pattern. BLI can provide protein binding and quantitative information, as well as kinetic information about the affinity and stability of the interaction.

Figure 1. Biolayer Interferometry (BLI) technology assay principle (Concepcion, J.; et al. 2009)

Selection of Biosensors

When BLI is applied to kinetic constant determination experiments, commonly used biosensors mainly include SA, SSA, AR2G, APS, AHC and AMC, etc. Each biosensor is applicable to different biological samples, and the requirements for the use of different biosensors are also different. For example, SSA is only suitable for detecting small molecule samples. SA is used to cure biotinylated proteins, antibodies, compounds and nucleic acids to detect large molecules interacting with them, while AHC can directly cure human antibodies to detect interacting molecules.

BLI Service in Creative Proteomics

Creative Proteomics has been focusing on the research of molecular interactions for nearly two decades. The rich experience and outstanding insights have made us a biotechnology company trusted by researchers all over the world. The BLI technology platform we established is jointly managed by professional BLI technicians and molecular biologists. Our professionalism and dedication have won us a lot of praise.

• Our platform includes FortéBioOctet RED384 and BLItz instruments. Customers can choose specific equipment according to project requirements (sample size, molecular size, speed, etc.). Our professional technicians can guarantee the accuracy of the experiment.
• This platform can conduct various researches, including protein quantification, kinetic determination, affinity determination, drug/antibody screening, drug development, etc. It can accurately and sensitively provide Ka, Kd ((kinetic binding), KD (equilibrium binding), and other data.

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Advantages of Biolayer Interferometry Service

Real-time Kinetic Data Acquisition: Enables dynamic data measurement for real-time detection of molecular interactions, including transient interactions.

Unique High-Throughput Platform: Capable of simultaneously analyzing 8 to 16 samples, making it the highest-throughput option among label-free technologies.

Minimal Sample Requirement: Requires only a small amount of nanomoles of samples, suitable for challenging molecular samples.

Wide Applicability: Directly analyzes crude samples, ensuring robust performance in various solution environments with selective detection.

High Sensitivity and Low Noise: Exhibits high sensitivity for accurate results in molecular interaction studies while minimizing vibration and mechanical noise.

Short Experimental Duration: Facilitates quick experimentation, ensuring efficient data collection.

Creative Proteomics is an international biotechnology company dedicated to the study of intermolecular interactions and other related fields. We have established an advanced biolayer interferometry technology platform that can supplement label-dependent methods to help customers study molecular interactions fast and in real time.