CLIP-Seq Service

CLIP-seq (crosslinking-immunoprecipitation and high-throughput sequencing), also known as HITS-CLIP, is a revolutionary technique to reveal the interaction of RNA molecules with RNA binding proteins (RBPs) at the genome-wide level. CLIP-seq can be used to study the binding mode of RNA Binding protein (RBP) to many RNA targets in vivo by high throughput, and reveal the function in some important biological processes. It can also be used for miRNA target identification, lncRNA biological function study, etc.

Creative Proteomics offers CLIP-seq services for the detection of various targets, RNA-binding proteins, RBP-RNA complexes, and circRNA-microRNA interactions. We have experience in providing analysis for a wide range of species, including animals (human, mouse, rat, human, sheep, cow, chicken, pig, fish, etc.), plants (Arabidopsis, rice, cotton, wheat, potato, tomato, apple, grape, cabbage, alfalfa dicotyledon, etc.), microorganisms (E. coli, Fusarium graminearum, Vibrio, etc.), and complex tissues (e.g. potato tubers, adipocytes).

Principle of CLIP-Seq

The main principle of CLIP-seq is based on the coupling of RNA molecules to RNA-binding proteins under UV irradiation. Antibodies specific for the RNA-binding protein precipitate the RNA-protein complex and then recover the RNA fragments from it. These molecules are subjected to high-throughput sequencing after steps such as addition of connectors and RT-PCR. Through bioinformatics analysis and processing and summarization, specific patterns are mined to reveal the regulatory roles of RNA-binding proteins and RNA molecules and their significance to life in depth.

Library Construction of CLIP-Seq

Experimental Procedure of CLIP-Seq

Advantages of CLIP-Seq

• Transcriptome-wide study of RNA-binding proteins and RNA interaction networks
• In situ study of RNA-binding proteins and RNA interactions in living cells
• UV cross-linking enhances RNA binding strength, allowing identification of short sequences that actually bind
• Self-developed analysis process with unique Peak calling technology to provide personalized, multi-omics association analysis to help resolve target protein regulatory mechanisms

Creative Proteomics has accumulated a wealth of experience in RNA-protein interaction research. Our professional technical team can provide efficient CLIP-seq and many related features to our customers. Contact us to learn more.