In Situ Binding Assay of Virus-Host Interaction Analysis
Background
Viruses are obligate intracellular parasites that have co-evolved with their hosts for millions of years, and their life activities depend on the close interactions with host cells. After the virus recognizes the target cell, it begins to use its specialized intracellular environment to replicate, this process affects cell function and may cause disease. Therefore, the study of virus-host interaction is essential for understanding these disease states and developing effective treatments.
Currently, there are many methods that can be used for virus-host interaction analysis to help us explore the interaction network, but what we need to know is that no matter which method, there are limitations in a specific environment. Therefore, it is necessary to combine multiple methods for the study of these interactions. Before designing an experiment, we should fully understand the nature of the experiment and conduct a comprehensive analysis.
Services
In contrast to ex situ binding assays, in situ assays are measured in cells, so the number of artificial interactions is limited, and the modification on the protein may be closer to the state during infection. Creative Proteomics offers a variety of in situ binding assays, including but not limited to affinity purification-mass spectrometry (AP-MS) and proximity-dependent labeling (PDL).
We offer multiple types of AP-MS, including tandem AP-MS, native AP-MS, GFP tagged AP-MS, etc., and the biotinylation based BioID system.
Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.
Applications
- Virus-host interaction research
- Pathogenic mechanism research
- Virus life cycle research
- Antiviral drug development
- Develop new avenues of patient care
Highlights
- Fast and highly accurate
- Able to screen large numbers of bait and prey combinations simultaneously
- Experimental guidance provided by experienced experts
- Tailored experimental program
Creative Proteomics has combined a variety of advanced technologies to establish a mature virus-host interaction analysis platform. In this platform, experiments can be conducted through various methods including in situ and ex situ, which saves customers time and effort while obtaining high-quality data. We are honored to be your competent research assistant.
References
- Norval, M. Virus-cell interactions. Medical Microbiology. 2012: 82-96.
- Gillen, J.; Nita-Lazar, A. Experimental analysis of viral-host interactions. Frontiers in Physiology. 2019, 10.
