DNA/RNA-Drug Interaction Analysis

Background

DNA and RNA play an important role in various biological processes, they are involved in gene storage, replication, transcription and many other essential life activities. Therefore, targeted nucleic acids can widely regulate biological processes, especially genetic diseases. Drugs that bind nucleic acids have been proven to be effective against cancer and viruses, affecting the health of millions of people worldwide. The interaction between drugs and nucleic acids can directly affect DNA replication, or cause strand breaks and mutations, and can also cause chemical and conformational modifications, thereby changing the electrochemical properties of nucleobases. Therefore, drugs that target nucleic acids have become a hot topic in recent years. People need to make detailed studies on the interaction between drugs and nucleic acids to clarify the mechanism of drug action, and ultimately design drugs that accurately target nucleic acids.

However, due to the complexity and flexibility of nucleic acids, the design and discovery of drugs targeting nucleic acids are more difficult than targeting proteins. After Creative Proteomics realized these problems, we vigorously developed structural biology platforms, computational chemistry and electrochemical technologies, etc., to obtain qualitative or quantitative information on the interaction between drugs and nucleic acids.

Fig 1. Three-dimensional structure diagram of the complex of distamycin (orange) and DNA duplex (Hasanzadeh, M.; Shadjou, N. 2106)

Services

The characterization of nucleic acid-drug interactions usually requires a combination of chemical, physical, and biological techniques, and finally obtain dissociation constants, stoichiometry, kinetic constants, and structural information to evaluate the degree and properties of specific types of interactions bound to nucleic acids. The nucleic acid-drug interaction platform developed by Creative Proteomics includes but is not limited to the following technologies.

• Mixture-based methods

Including nuclear magnetic resonance (NMR), surface plasmon resonance (SPR), ultraviolet absorption, Raman spectroscopy, mass spectrometry (MS), calorimetry, etc.

• Separation-based methods

Include chromatography (liquid chromatography and thin-layer chromatography), dialysis, ultracentrifugation, and electrophoresis (planar and capillary electrophoresis), etc.

• Electrochemical technology

It is based on the use of electrodes to detect changes in these molecules. After the interaction, the voltammetric signal intensity and position of the drug and nucleic acid may change. Electrochemical technology can be combined with spectroscopy to expand the scope of research to obtain useful insights into the molecular mechanism of nucleic acid-drug interactions.

Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.

Applications

• Discovery and development of anticancer and antiviral drugs
• Research on the drug action mechanism
• Disease pathogenesis research
• Nucleic acid-drug interaction research

Highlights

• High sensitivity and high selectivity
• Combine numerous technologies
• Professional data processing and calculation tools
• One-stop service

Creative Proteomics has been committed to promoting the development of molecular interaction and providing high-quality analysis services to customers in this industry. We uphold a rigorous attitude to ensure the accuracy and repeatability of experimental results. We are cost-effective and can also provide emergency services to customers.